File upload and parameters selection

Select analysis model

Parameters for ncRNA detection (single sample)

Parameters for ncRNA detection (multiple samples)

Parameters for difference test (paired samples)

To detect differentially expressed small RNAs in paired samples, two statistical test methods were used by CPSS. When the counts of the tag are smaller than 5, Fisher's exact test will be used for judgment. A chi-square test will be used if the counts is more than 5.Additionally, the expression of each tag was normalized based on the absolute number of mappable reads (Normalized counts=Reads per Million). The fold change was calculated based on the normalized value.

Parameters for difference test (group samples)

For group samples, Kolmogorov–Smirnov test is used to generate P-value. Fold change is calculated using the same method above.

Parameters for functional analysis

Queue

Job is enqueued

Start analysis

General results

Genome mapping results

Length distribution of genome mapped reads

Chromosome distribution of genome mapped reads

miRNA family matching results

ncRNA matching results

Difference test

Samples difference test

Groups difference test

Detailed results

Details of ncRNA expression

Details of predicted novel miRNA

Functional analysis

miRNA target prediction

GO analysis

Pathway analysis

Protein domian analysis

Protein-protein interaction (PPI) analysis